Automated nucleic acid extraction

Analytik Jena | Life Science offers a wide range of kits for automated magnetic beads based nucleic acid extraction. We offering kits not only for our system InnuPure® C12 but also for the KingFisher® systems. All available kits will be delivered ready-to-use with all necessary solutions and plastics for minimal handling steps. That will surely save your time and money. Just give this a try.

 

bs_automat_NSA

For direct isolation of genomic DNA up to 200 µl of whole blood samples, including integration of lysis step in the automatic extraction process using the InnuPure® C12.

 

Product specifications:

1. Starting material

  • Fresh or frozen whole blood samples stabilized with EDTA or citrate (200 µl)
  • Special protocol for old blood samples (e.g. stabilized with 4 M Urea; 200 µl)

2. Time for isolation
Lysis:
for fresh samples: automatic on device
for old samples: approx. 10 min
InnuPure® C12 protocol
fresh samples: approx. 47 min
old samples: approx. 25 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP Blood DNA Mini Kit – IP should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C! Store dissolved
Proteinase K at –20°C. Repeated freezing and thawing will reduce the activity dramatically.

Kit components:
Lysis Solution, Proteinase K, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-1012012 12 reactions
845-IP-1012096 96 reactions
845-IP-1012480 480 reactions
 

For isolation of total RNA from eucaryotic cells using InnuPure® C12. The extraction procedure is based on lysis of starting material and subsequent selective removing of genomic DNA using a Spin Filter prefiltration step. After these steps all other steps are runing automatically on the InnuPure® C12 based on binding of the total RNA on magnetic particle, washing of the bound RNA and eluting of the RNA.

 

Product specifications:

1. Starting material

  • Eucaryotic cells (5 x 106)
  • gram+ and gram- bacteria (1 x 109)

2. Time for isolation
Lysis:                  
approx. 10 – 15 min
InnuPure® C12 protocol:  
approx. 20 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP Cell RNA Kit - IP should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Please store MAG Suspension at 4°C.

Kit components:
Lysis Solution, Spin Filter, Receiver Tubes, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-4012012 12 reactions
845-IP-4012096 96 reactions
845-IP-4012480 480 reactions
 

For isolation of total RNA from tissue sample using InnuPure® C12. The extraction procedure is based on lysis of starting material and subsequent selective removing of genomic DNA using a Spin Filter prefiltration step. After these steps all other steps are runing automatically on the InnuPure® C12 based on binding of the total RNA on magnetic particle, washing of the bound RNA and eluting of the RNA.

 

Product specifications:

1. Starting material
Different kinds of tissue samples (up to 20 mg)

2. Time for isolation
Homogenization:
Homogenizer:  approx. 30 sec – 2 x 2 min
Liquid nitrogen: approx. 5 – 10 min
Lysis:                 
approx. 20 min
InnuPure® C12 protocol:  
approx. 20 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP Tissue RNA Kit - IP should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Please store MAG Suspension at 4°C.

Kit components:
Lysis Solution, Spin Filter, Receiver Tubes, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-4112012 12 reactions
845-IP-4112096 96 reactions
845-IP-4112480 480 reactions
 

For isolation of total RNA from plant sample using InnuPure® C12. The extraction procedure is based on lysis of starting material and subsequent selective removing of genomic DNA using a Spin Filter prefiltration step. After these steps all other steps are runing automatically on the InnuPure® C12 based on binding of the total RNA on magnetic particle, washing of the bound RNA and eluting of the RNA.

 

Product specifications:

1. Starting material
Different kinds of plant material (up to 50 mg)

2. Time for isolation
Homogenization:
Homogenizer:        approx: 1 – 3 min
Liquid nitrogen:    approx: 5 – 10 min
Lysis:                  
approx: 15 min
InnuPure® C12 protocol:  
approx: 20 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP Plant RNA Kit - IP should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Please store MAG Suspension at 4°C.

Kit components:
Lysis Solutions, Spin Filter, Receiver Tubes, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-4212012 12 reactions
845-IP-4212096 96 reactions
845-IP-4212480 480 reactions

 

 

For isolation of DNA from pellets of  gram+ and gram– bacteria after cultivation using the InnuPure® C12.

 

Product specifications:

1. Starting material

  • Bacterial cell pellets after cultivation (max. 1 x 109 cells)
  • Bacterial cells from tissue samples (max. 30 mg tissue)

2. Time for isolation
Lysis:                  
approx. 25 – 30 min
InnuPure® C12 protocol:  
approx: 17 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP Bacteria DNA Kit - IP should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C.
Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, Proteinase K, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-5512012 12 reactions
845-IP-5512096 96 reactions
845-IP-5512480 480 reactions
 

For isolation of mycobacteria DNA from sputum or BALB sample using InnuPure® C12.

 

Product specifications:

1. Starting material

  • Bacterial cell pellets after cultivation (max. 1 x 109 cells)
  • Sputum samples (200 µl)
  • Bronchoalveolar lavage samples (up to 1.0 ml)

2. Time for isolation
Lysis:     
Bacterial cell pellets: approx. 55 – 65 min
Sputum samples:  approx. 105 min
Bronchoalveolar lavage: approx. 60 – 75 min
InnuPure® C12 protocol: approx. 20 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP Mycobacteria DNA Kit - IP should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C.
Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, Proteinase K, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-5812012 12 reactions
845-IP-5812096 96 reactions
845-IP-5812480 480 reactions
 

For isolation of viral DNA from serum, plasma or other cell-free body fluids, from tissue samples, paraffin embedded samples, swabs and from cell cultures using the InnuPure® C12.

 

Product specifications:

1. Starting material

  • Serum (220 µl)
  • Plasma (220 µl)
  • Cell culture supernatants (220 µl)
  • Cell-free body fluids (220 µl)

2. Time for isolation
Lysis: approx. 15 min
InnuPure® C12 protocol: approx. 17 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The INSTANT Virus DNA Kit - IP should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C.Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Extraction Tubes, Lysis Solution, Proteinase K, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-5312012 12 reactions
845-IP-5312096 96 reactions
845-IP-5312480 480 reactions
 

For direct automatic isolation of viral RNA from serum, plasma or other cell-free body fluids (automatic extraction process integrated the initial lysis of starting material) using the InnuPure® C12.

 

Product specifications:

1. Starting material

  • Serum (220 µl)
  • Plasma (220 µl)
  • Cell culture supernatants (220 µl)
  • Cell-free body fluids (220 µl)

2. Time for isolation
Lysis: automatic on InnuPure® C12
InnuPure® C12 protocol: approx. 35 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The INSTANT Virus RNA Kit - IP should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Extraction Tubes, Lysis Solution, Reagent Pack A, Reagent Pack B, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-5412012 12 reactions
845-IP-5412096 96 reactions
845-IP-5412480 480 reactions
 

For isolation of DNA from plant sample using InnuPure® C12. The extraction procedure is based on lysis of starting material and subsequent selective removing removing of proteines and polysaccharides. After these steps all other steps are runing automatically on the InnuPure® C12 based on binding of the genomic DNA on magnetic particle, washing of the bound DNA and eluting of the DNA.

 

Product specifications:

1. Starting material

  • Plant samples fresh or frozen (max. 100 mg)
  • Plant samples with a high percentage of water (max. 150 mg)

2. Time for isolation
Homogenization:
Homogenizer:        approx: 1 – 3 min
Liquid nitrogen:    approx: 5 – 10 min
Lysis:                        approx. 40 – 45 min
InnuPure® C12 protocol:        approx: 19 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.
Positive tested for: parsley, carrots, potatoes, seeds (e.g. soy and maize)

Storage conditions:

The innuPREP Plant DNA Kit - IP should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store MAG Suspension at 4°C!
Invert the Reagent Pack A and the Reagent Pack B for 3 – 4 times and thump it onto a table to collect the pre-filled solutions at the bottom of the single strips.

Kit components:
Lysis Solution, Precipitation Buffer, Reagent Pack A, Reagent Pack B, MAG Suspension, Pre-Filter, MFR Tips, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-IP-1512012 12 reactions
845-IP-1512096 96 reactions
845-IP-1512480 480 reactions
 

For isolation of  DNA from different  types of forensic starting materials like tissue sample, buccal swabs, swabs, blood or blood stains, stamps and envelops, hair roots and hairs, finger nails, sperm stains, fingerprints etc. using the InnuPure® C12. The kit contains special developed Lysis Tube with preformulated dried storage stable lysis reagents. No addition of Lysis Solution or proteolytic enzymes is necessary.

 

Product specification:

1. Starting material
Swabs from different surfaces (e.g. cups, bottles, fingerprints)
Blood samples
Sperm samples
Hair, hair roots or barb hairs
Envelopes
Finger nails
Cigarette butts or paper
Chewing gum
Tissue samples

2. Time for isolation
Lysis:         
Protocol 1    approx. 15 – 20 min
Protocol 2    approx. 120 min
Protocol 3    approx. 120 min
Protocol 4    approx. 60 min
InnuPure® C12 protocol: approx. 17 min

3. Typical yield:
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP Forensic Easy Kit - IP should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Please store MAG Suspension at 4°C.

Kit components:
PLP Tube, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR-Tips, Pre-filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-IP-2512012 12 reactions
845-IP-2512096 96 reactions
845-IP-2512480 480 reactions
 

For the very fast and efficient isolation of high yield genomic DNA from buccal swabs. The recovery of DNA and the quality are excellent. The kit contains also swabs for sample collecting and special Lysis Tubes PLP. This tubes contain solid formula of proteolytic reagents. No reagents must be added for the lysis step.

 

Product specifications:

1. Starting material
Buccal swab samples

2. Time for isolation
Lysis: approx. 15 min
InnuPure® C12 protocol: approx. 17 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:The innuPREP Swab DNA easy Kit - IP should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Please store MAG Suspension at 4°C.

Kit components:
PLP Tubes, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Swabs, Elution Tubes, Manual

Order-No.
 Amount
845-IP-2312012 12 reactions
845-IP-2312096 96 reactions
845-IP-2312480 480 reactions
 

For isolation of genomic DNA up to 2 ml of whole blood samples using the InnuPure® C12.

 

Product specifications:

1. Starting material
Fresh or frozen whole blood samples stabilized with EDTA or citrate (0.5 – 2 ml)

2. Time for isolation
Lysis:
for up to 1 ml: approx. 40 – 45 min
for up to 2 ml: approx. 50 – 55 min
InnuPure® C12 protocol:  
approx. 22 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP Blood DNA Midi Kit – IP should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Ery Lysis Solution, PLP Tubes, Reagent Pack A, Reagent Pack B, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-1212012 12 reactions
845-IP-1212096 96 reactions
845-IP-1212480 480 reactions
 

For isolation of genomic DNA from different types of starting material like tissue sample, buccal swabs, eucaryotic cells using the InnuPure® C12.

 

Product specifications:

1.Starting material

  • Tissue samples (max. 30 mg)
  • Viscous tissue samples (e.g. Spleen, max. 10 mg)
  • Rodent tails (0.5 cm)
  • Paraffin embedded tissue samples
  • Buccal swab samples

2. Time for isolation
Lysis:  
Tissue and tails: approx. 45 – 50 min
Paraffin samples: approx. 130 min
Buccal swabs: approx. 15 min
InnuPure® C12 protocol: 
approx: 17 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material

Storage conditions:
The innuPREP DNA Kit - IP should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, Proteinase K, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-2012012 12 reactions
845-IP-2012096 96 reactions
845-IP-2012480 480 reactions
 

For the very fast and efficient isolation of high yield genomic DNA from buccal swabs. The recovery of DNA and the quality are excellent. The kit contains also swabs for sample collecting.

 

Product specifications:

1. Starting material
Buccal swab samples

2. Time for isolation
Lysis: approx: 15 min
InnuPure® C12 protocol: approx: 17 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP Swab DNA Kit - IP should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, Proteinase K, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Swabs, Elution Tubes, Manual

Order-No.
 Amount
845-IP-2112012 12 reactions
845-IP-2112096 96 reactions
845-IP-2112480 480 reactions
 

For isolation of bacterial DNA or host DNA from stool samples using InnuPure® C12.

 

Product specifications:

1. Starting material
Fresh or frozen stool samples (200 - 300 mg or 200 - 300 µl)

2. Time for isolation
Homogenization: approx. 3 – 4 min
Lysis: approx. 25 min
InnuPure® C12 protocol: approx. 19 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP Stool DNA Kit - IP should be stored dry, at room temperature (14°C– 25 °C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Please store MAG Suspension at 4°C.

Kit components:
Lysis Solution, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Pre-filter, Lysis Tube, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-IP-3012012 12 reactions
845-IP-3012096 96 reactions
845-IP-3012480 480 reactions
 

For isolation of DNA from different types of forensic starting materials like tissue sample, buccal swabs, swabs, blood or blood stains, stamps and envelops, cigarette butts, hair roots and hairs, finger nails, sperm stains, fingerprints etc. using the InnuPure® C12.

 

Product specifications:

1. Starting material

  • Swabs from different surfaces (e.g. cups, bottles, fingerprints)
  • Blood samples
  • Sperm samples
  • Hair, hair roots or barb hairs
  • Envelopes
  • Finger nails
  • Cigarette butts or paper
  • Chewing gum
  • Tissue samples

2. Time for isolation
Lysis:
Protocol 1    approx. 15 – 20 min
Protocol 2    approx. 120 min
Protocol 3    approx. 120 min
Protocol 4    approx. 60 min
InnuPure® C12 protocol:  
approx. 17 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP Forensic Kit - IP should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, Proteinase K, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-2412012 12 reactions
845-IP-2412096 96 reactions
845-IP-2412480 480 reactions
 

For isolation of viral DNA from serum, plasma or other cell-free body fluids; from tissue samples, paraffin embedded samples, swabs and from cell cultures using the InnuPure® C12.

 

Product specifications:

1. Starting material

  • Serum (200 µl)
  • Plasma (200 µl)
  • Cell culture supernatants (200 µl)
  • Cell-free body fluids (200 µl)

2. Time for isolation
Lysis: approx. 15 min
InnuPure® C12 protocol:approx. 20 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP Virus DNA Kit - IP should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, Proteinase K, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-5112012 12 reactions
845-IP-5112096 96 reactions
845-IP-5112480 480 reactions
 

For direct automatic isolation of viral RNA from serum, plasma or other cell-free body fluids (automatic extraction process integrated the initial lysis of starting material) using the InnuPure® C12.

 

Product specifications:

1. Starting material

  • Serum (200 µl)
  • Plasma (200 µl)
  • Cell culture supernatants (200 µl)
  • Cell-free body fluids (200 µl)

2. Time for isolation
Lysis: automatic on InnuPure® C12
InnuPure® C12 protocol: approx. 35 min

3. Typical yield
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP Virus RNA Kit - IP should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Reagent Pack A, Reagent Pack B, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-5212012 12 reactions
845-IP-5212096 96 reactions
845-IP-5212480 480 reactions
 

For isolation of genomic DNA from different types of starting material like tissue sample, buccal swabs, eucaryotic cells using the InnuPure® C12. This kit contains special Lysis Tubes PLP. This tubes contain solid formula of proteolytic reagents. No reagents must be added for the lysis step.

 

Product specifications:

1. Starting material:

  • Tissue samples (max. 30 mg)
  • Viscous tissue samples (e.g. Spleen, max. 10 mg)
  • Rodent tails (0.5 cm)
  • Paraffin embedded tissue samples
  • Buccal swab samples

2. Time for isolation:
Lysis: 
Tissue and tails: approx. 45 – 50 min
Paraffin samples: approx. 130 min
Buccal swabs: approx. 15 min
InnuPure® C12 protocol: approx. 17 min

3. Typical yield:
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The innuPREP DNA easy Kit - IP should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Please store MAG Suspension at 4°C.

Kit components:
PLP Tubes, Reagent Pack , Reagent Pack B, MAG Suspension, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-2212012 12 reactions
845-IP-2212096 96 reactions
845-IP-2212480 480 reactions
 

For isolation of DNA from pellets of  gram+ and gram– bacteria after cultivation using the InnuPure® C12.

 

Product specifications:

1. Starting material:

  • Bacterial cell pellets after cultivation (max. 1 x 109 cells)
  • Bacterial cells from tissue samples (max. 30 mg tissue)

2. Time for isolation:
Lysis:
approx. 25 – 30 min
InnuPure® C12 protocol:
approx.17 min

3. Typical yield:
Not determined. The yield depends on the type and the amount of the starting material.

Storage conditions:
The INSTANT Bacteria DNA Kit - IP should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C.
Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Extraction Tubes, Lysis Solution, Proteinase K, Reagent Pack A, Reagent Pack B, MAG Suspension, MFR Tips, Elution Tubes, Manual

Order-No.
 Amount
845-IP-5612012 12 reactions
845-IP-5612096 96 reactions
845-IP-5612480 480 reactions
 

For isolation of genomic DNA from whole blood and tissue sample and for isolation of bacterial DNA from urine and urogenital swabs using KingFisher® mL processor.

 

Storage conditions:
The innuPREP DNA Kit I – KFml should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, MAG Suspension, Proteinase K, Washing Solutions, Elution Buffer, KingFisher Tip, KingFisher Tube Strips, Manual

Order-No. Amount
845-KF-8015015 15 reactions
845-KF-8015250 250 reactions
845-KF-8015750 750 reactions
 

For very fast and efficient isolation of BVDV RNA, Influenca RNA and Swine Fever RNA  (45 minutes protocol) from serum or plasma sample using KingFisher® 96 Flex processor.

 

Order-No.

 Amount
845-KF-4996096 1 x 96 reactions
845-KF-4996480 5 x 96 reactions


Further information will follow soon.

 

For very fast and efficient isolation of BVDV RNA (45 minute protocol) from serum or plasma sample using KingFisher® 96 Flex processor.

 

Order-No.

 Amount
845-KF-4796096 1 x 96  reactions
845-KF-4796480 5 x 96 reactions


Further information will follow soon.

 

 For isolation of ds BTV RNA from whole blood sample using KingFisher® 96 Flex processor.

 

Order-No.

 Amount
845-KF-4896096 1 x 96  reactions
845-KF-4896480 5 x 96 reactions


Further information will follow soon.

 

For isolation of viral DNA and RNA from serum, plasma or other cell free body fluids; from tissue samples, paraffin embedded samples, swabs,  and from cell cultures using KingFisher® 96 Flex processor.

 

Order-No.

 Amount
845-KF-4696096 1 x 96 reactions
845-KF-4696480 5 x 96 reactions


Further information will follow soon.

 

For isolation of genomic or viral DNA from whole blood samples using KingFisher® 96 Flex processor.

 

Order-No.

 Amount
845-KF-8096096 1 x 96  reactions
845-KF-8096480 5 x 96  reactions


Further information will follow soon.

 

Kit for isolation of genomic DNA from tissue sample or rodent tails using KingFisher® 96 magnetic particle processor.

 

Order-No.

 Amount
845-KF-7115096 1 x 96  reactions
845-KF-7115480 5 x 96 reactions


Further information will follow soon.

 

For isolation of bacterial DNA from stool samples using KingFisher® 96 processor.

 

Order-No.

 Amount
845-KF-7015096 1 x 96 reactions
845-KF-7015480 5 x 96 reactions


Further information will follow soon.

 

For isolation of ds BTV RNA from whole blood sample using KingFisher® mL processor.The kit contains Extraction Tube coated with storage stable Internal Extraction Tubes and Carier nucleic acids.

 
Order-No.
 Amount
845-KF-4515015 15 reactions
845-KF-4515250 250 reactions
845-KF-4515750 750 reactions


Furthe information will follow soon.

 

For isolation of viral RNA from serum, plasma or other cell free body fluids; from tissue samples, paraffin embedded samples, swabs and from cell cultures using KingFisher® mL processor.The kit contains Extraction Tube coated with storage stable Internal Extraction Tubes and Carier nucleic acids.

 

Storage conditions:
The INSTANT Virus RNA Kit – KFml should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Please store the MAG Suspension at 4°C – 8°C.

Kit components:
Extraction Tubes, MAG Suspension, Lysis Solution, Binding Solution, RNase-free water, Washing Solutions, Elution Tubes, KingFisher Tip Combs, KingFisher Tube Strips, Manual

Order-No.
 Amount
845-KF-4215015 15 reactions
845-KF-4215250 250 reactions
845-KF-4215750 750 reactions
 

For isolation of viral DNA from serum, plasma or other cell free body fluids, from tissue samples, paraffin embedded samples, swabs and from cell cultures using KingFisher® mL processor. The kit contains Extraction Tube coated with storage stable Internal Extraction Tubes and Carier nucleic acids.

 

Storage conditions:
The INSTANT Virus DNA Kit – KFml should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Extraction Tubes, MAG Suspension, Lysis Solution, Binding Solution, Proteinase K, Washing Solutions, Elution Buffer, Spin Filter, KingFisher Tip Combs, KingFisher Tube Strips, Manual

Order-No.
 Amount
845-KF-4115015 15 reactions
845-KF-4115250 250 reactions
845-KF-4115750 750 reactions
 

For isolation of ds BTV RNA from whole blood sample using KingFisher® mL processor.

 
Order-No.
 Amount
845-KF-4815015 15 reactions
845-KF-4815250 250 reactions
845-KF-4815750 750 reactions


Further information will follow soon.

 

For isolation of viral DNA and RNA from serum, plasma or other cell free body fluids; from tissue samples, paraffin embedded samples, swabs and from cell cultures using KingFisher® mL processor.

 
Order-No.
 Amount
845-KF-4615015 15 reactions
845-KF-4615250 250 reactions
845-KF-4615750 750 reactions


Further information will follow soon.

 

For isolation of viral RNA from serum, plasma or other cell free body fluids; from tissue samples, paraffin embedded samples, swabs and from cell cultures using KingFisher® mL processor.

 
Order-No.
 Amount
845-KF-4515015 15 reactions
845-KF-4515250 250 reactions
845-KF-4515750 750 reactions


Further information will follow soon.

 

For isolation of viral DNA from serum, plasma or other cell free body fluids, from tissue samples, paraffin embedded samples, swabs and from cell cultures using KingFisher® mL processor.

 
Order-No.
 Amount
845-KF-4715015 15 reactions
845-KF-4715250 250 reactions
845-KF-4715750 750 reactions


Further information will follow soon.

 

For isolation of DNA from pellets of gram+ and gram- bacteria after cultivation using KingFisher® mL processor.

 
Order-No.
 Amount
845-KF-6015015 15 reactions
845-KF-6015250 250 reactions
845-KF-6015750 750 reactions


Further information follow soon.

 

For isolation of genomic DNA from whole blood sample using KingFisher® mL processor.

 
Order-No.
 Amount
845-KF-8115015 15 reactions
845-KF-8115250 250 reactions
845-KF-8115750 750 reactions


Further information follow soon.

 

For isolation of viral from serum, plasma or other cell free body fluids; from tissue samples, paraffin embedded samples, swabs, stool sample and fromcell cultures using KingFisher® 96 Flex processor.

 

Order-No.

 Amount
845-KF-4596096 1 x 96 reactions
845-KF-4596480 5 x 96 reactions


Further information will follow soon.